Intestinal absorption of amino acids by rainbow trout,Salmo gairdneri (Richardson) |
| |
Authors: | L Ingham and C Arme |
| |
Institution: | (1) Department of Zoology, Queen's University, Belfast, Northern Ireland;(2) Present address: Biology Department, North Staffordshire Polytechnic, Stoke-on-Trent, Staffs., England |
| |
Abstract: | Summary A technique for the in vitro maintenance of isolated portions of rainbow trout intestine is described. Uptake of14C-L-leucine occurs by an active mechanism which is stereospecific, sodium-dependent and susceptible to inhibition by other neutral amino acids.K
t
for leucine uptake is 2.72 mM with aV
max of 19.61 moles/g ethanol extracted dry wt.·10 min. L-valine and L-methionine are competitive inhibitors of L-leucine uptake withK
i
values of 24.30 mM and 2.56 mM, respectively. Evidence suggests that at least two uptake sites for the transport of neutral amino acids are present in the intestine of this species. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|