Interacting proteins Rtt109 and Vps75 affect the efficiency of non-homologous end-joining in Saccharomyces cerevisiae |
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Authors: | Jessulat Matthew Alamgir Md Salsali Hamid Greenblatt Jack Xu Jianhua Golshani Ashkan |
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Institution: | a Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, Ont., Canada K1S 5B6 b Banting and Best Department of Medical Research, University of Toronto, Toronto, Canada M5G 1L6 c Ottawa Institute of Systems Biology, Ottawa, Canada |
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Abstract: | One of the key pathways for DNA double-stranded break (DSB) repair is the non-homologous end-joining (NHEJ) pathway, which directly re-ligates two broken ends of DNA. Using a plasmid repair assay screen, we identified that the deletion strain for RTT109 had a reduced efficiency for NHEJ in yeast. This deletion strain also had a reduced efficiency to repair induced chromosomal DSBs in vivo. Tandem-affinity purification of Rtt109 recovered Vps75 as a physical interacting protein. Deletion of VPS75 was also shown to have an effect on the efficiency of NHEJ in both the plasmid repair and the chromosomal repair assays. In addition, deletion mutants for both RTT109 and VPS75 showed hypersensitivity to different DNA damaging agents. Our genetic interaction analysis supports a role for RTT109 in DNA damage repair. We propose that one function of the Rtt109-Vps75 interacting protein pair is to affect the efficiency of NHEJ in yeast. Vps75 but not Rtt109 also seem to have an effect on the efficiency of DSB repair using homologous recombination. |
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Keywords: | DNA repair Novel gene function NHEJ Double-stranded break Protein-protein interaction Homologous recombination |
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