Root Morphology and Gene Expression Analysis in Response to Drought Stress in Maize (Zea mays) |
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Authors: | Tingbo Jiang Jake Fountain Georgia Davis Robert Kemerait Brian Scully R Dewey Lee Baozhu Guo |
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Institution: | (1) Department of Plant Pathology, University of Georgia, Tifton, GA, USA;(2) Key Laboratory of Forest Tree Genetic Improvement and Biotechnology of Ministry of Education, Northeastern Forestry University, Harbin, China;(3) Agricultural Research Service, Crop Protection and Management Research Unit, USDA, Tifton, GA, USA;(4) Department of Plant Pathology and Physiology, Louisiana State University, Baton Rouge, LA, USA;(5) Division of Plant Sciences, University of Missouri–Columbia, Columbia, MO, USA;(6) Department of Crop and Soil Sciences, University of Georgia, Tifton, GA, USA; |
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Abstract: | Water-deficit stress tolerance is a complex trait, and water deficit results in various physiological and chemical changes
in maize (Zea mays L.) and exacerbates pre-harvest aflatoxin contamination. The objective of this study was to characterize the variations in
morphology, physiology, and gene expression in two contrasting inbred lines, Lo964 and Lo1016, in order to understand the
differences in response to water-deficit stress. The results revealed that Lo964 was less sensitive to water-deficit stress,
and had a strong lateral root system and a higher root/shoot ratio in comparison to Lo1016. In response to water-deficit stress
by comparing stressed versus well-watered conditions, abscisic acid syntheses were increased in leaves, roots, and kernels
of both Lo964 and Lo1016, but by different magnitudes. Indole-3-acetic acid (IAA) was undetectable in the leaves and roots
of either genotype regardless of treatments, but increases of 58% and 8% in IAA concentration were observed in 20 DAP kernels,
in response to water-deficit stress, respectively. The expression of the MIPS was up-regulated 7-fold in leaf tissues of Lo964 compared to Lo1016 at watered conditions, but decreased significantly to
similar levels in both genotypes at water-deficit conditions. ZmPR10 and ZmFer1 expressions tended to up-regulate although ZmPR10 was expressed higher in root tissue while ZmFer1 was expressed higher in leaf tissue. Further study is needed to confirm if Lo964 has reduced aflatoxin contamination associated
with the drought tolerance in the field in order to utilize the resistant trait in breeding. |
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