Combined Aldehyde-Fuchsin and Periodic Acid-Schiff Staining of the Pituitary |
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Authors: | Herbert Elftman |
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Affiliation: | a Department of Anatomy, College of Physicians and Surgeons, Columbia University, New York 32, NY |
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Abstract: | Aldehyde-fuchsin and the periodic acid-Schiff procedure can be applied in sequence to the same tissue section and combined with a stain for acidophils, such as orange G, for routine analysis of the pituitary. This is made possible by fixation in a mixture containing 5% chrome-alum, 5% HgCl2 and 5% formalin; control of the pH and the activity of the aldehyde-fuchsin; and increased sensitivity of the Schiff reagent, effected by reducing its SO2 content. Although designed especially for the pituitary, the procedure is also applicable to other histological problems. |
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