摘 要: | LowTemperatureInductionofFreesiaCormsinVitroHUANGMin-Ling,ChENShi-Lin(OrnamentalHorticulturalLaboratory,GujianAcademyofAgriculturalSciences,Fuzhou350013)1植物名称小苍兰(Freesiahybrida)品种“Aurora”2材料类别茎尖。3塔共条件将萌芽的球茎剥去外皮,用70%洒精消毒5min,再用0.l%HgCI。加入数滴吐温一80溶液消毒10min,无菌水冲洗3次,切取0.5~0.8cm的茎尖。以MS为基本培养基,(互)例芽诱导及增殖培养基为MS+BA3~4mg/L(单位下同)+NAA0.2~0.4,光强1000!X,温度24士ZC。(2)诱导试…
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