酶降解法精制肝素钠

肝素钠在哺乳动物的脏器和体液如：肝、肺、脾及胃肠粘膜等中含量很高．肝素钠作为一种有效的抗凝剂已广泛应用于临床，生物学功能在于阻止血液中凝血酶的作用．其衍生物还具有降血脂、消炎、抗过敏、利尿及调节免疫功能等多种用途．肝素钠属于多糖类化合物，作为溶栓类药...

Purification of Heparin Sodium Salt by Enzymatic Degradation

There were some free proteins in crude product of the heparin sodium salt.The free proteins were difficult to remove using routine way in the purification of process.To remove the free proteins in the purification process of heparin sodium salt,the heparin sodium salt was degraded by trypsin in 50 mmol/L,pH 8 0 Tris HCl and degradation product was separated by DEAE Sepharose 4B anion exchange column.The specific activity of purified heparin sodium salt was about 170 U/mg.