An efficient protocol for genetic transformation of watercress (<Emphasis Type="Italic">Nasturtium officinale</Emphasis>) using <Emphasis Type="Italic">Agrobacterium rhizogenes</Emphasis> |
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Authors: | Nam?Il?Park Jae?Kwang?Kim Woo?Tae?Park Jin?Woong?Cho Email author" target="_blank">Yong?Pyo?LimEmail author Email author" target="_blank">Sang?Un?ParkEmail author |
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Institution: | (1) Department of Crop Science, College of Agriculture and Life Sciences, Chungnam National University, 220 Gung-dong, Yuseong-gu, Daejeon, 305-764, Korea;(2) National Academy of Agricultural Science, Rural Development Administration, Suwon, 441-707, Korea;(3) Department of Horticultural Science, College of Agriculture and Life Sciences, Chungnam National University, 220 Gung-dong, Yuseong-gu, Daejeon, 305-764, Korea; |
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Abstract: | Watercress (Nasturtium officinale) is a member of the Brassicaceae family and a rich source of glucosinolate, which has been shown to possess anticancer properties.
To extract these compounds from N. officinale for study, a method was developed in which Agrobacterium rhizogenes was used to transfer DNA segments into plant genomes in order to produce hairy root cultures, which are a reliable source
of plant compounds. The A. rhizogenes strain R1000 had the highest infection frequency and induces the most hairy roots per explant. Polymerase chain reaction
and cytohistochemical staining methods were used to validate transgenic hairy roots from N. officinale. Glucosinolate from watercress hairy roots was separated and analyzed using high-performance liquid chromatography coupled
to electrospray ionization mass spectrometry. Indolic glucosinolates, including glucobrassicin (0.01–0.02 μmol/g of DW) and
4-methoxyglucobrassicin (0.06–0.18 μmol/g of DW), as well as aromatic glucosinolate (gluconasturtiin) (0.06–0.21 μmol/g of
DW), were identified virtually identical or more in transformed than wild type roots of N. officinale. Hairy root culture of watercress is a valuable approach for future efforts in the metabolic engineering of glucosinolate
biofortification in plants, particularly, because indolic glucosinolates are the precursors of a potent cancer chemopreventive
agent (indole-3-carbinol). |
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