Study the polymorphism of CYP3A5 and CYP3A4 loci in Iranian population with laryngeal squamous cell carcinoma |
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Authors: | Negar Azarpira Mohamad Javad Ashraf Bigan Khademi Masumeh Darai Afsoon Hakimzadeh Elham Abedi |
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Institution: | (1) Organ Transplant Research Center, Nemazi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran;(2) Department of Pathology, Khalili Hospital, Shiraz University of Medical Sciences, Shiraz, Iran;(3) Department of Otolaryngology, Khalili Hospital, Shiraz University of Medical Sciences, Shiraz, Iran |
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Abstract: | Cancer reflects a complicated network of interactions between genes and environmental factors. Cytochrome P450 (CYP) is a
multi-gene superfamily participating in the metabolism of xenobiotics. The aim of our study was to examine whether polymorphisms
in the CYP enzyme genes affect the risk of developing larynx squamous cell carcinoma (SCC). Polymorphism of CYP3A5 and CYP3A4 genes were investigated in 50 patients with laryngeal SCC and 100 control subjects by polymerase chain reaction- restriction
fragment length polymorphism (PCR-RFLP). In patients the CYP3A5 3*/3* and 1*/3*genotypes were detected in 92% and 8% respectively. There was no relation between genotype, allele frequency and grade/stage
of tumor. In control group, the frequency of CYP3A5 3*/3* and CYP3A5 1*/3* genotype were 98% and 2% respectively. There was no significant difference in genotype and allele frequency of this gene
between patient and control group. In respect of CYP3A41A*/B*, people in both patient and control groups had the same genotype of CYP3A41A*/1A*. In this study, the CYP gene variants were not associated with increased risk of laryngeal SCC. Study on the other genetic
factors which are involved in activation/detoxication of procarcinogenes, such as CYP1A1, CYP1B1, CYP2E1 and gluthation S transferase is recommended. |
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