Separation of nucleic acid components on polyacrylamide gel columns.

The exclusion and sorptive properties of the polyacrylamide gel Bio-Gel P-2 for low-molecular-weight anionic species allows separations of nucleic acid components to be effected in dilute alkaline borate buffer at pH 8.8. The degree of ionization, determined by pH, fixes the elution position of the compounds chromatographed in this system. Optimum conditions are given for making group separations as well as many other useful separations of nucleic acid components and related compounds.