Characterization of somatic embryogenesis in sandalwood (Santalum album L.) |
| |
Authors: | K Sankara Rao N K Chrungoo Amares Sinha |
| |
Institution: | (1) Department of Biochemistry, Indian Institute of Science, 560 012 Bangalore, India;(2) Present address: Department of Botany, North-Eastern Hill University, 793 022 Shillong, India |
| |
Abstract: | In the synchronous embryogenesis system of sandalwood developed in our laboratory, we observed that the early events of differentiation
from freshly induced callus (stage 0) are accomplished in three distinct stages viz., preglobular masses (stage 1), globular
embryos (stage 2), and bipolar embryos (stage 3). Transition from stage 0 to 1 was accomplished using 2,4-D and involves a
stage specific appearance of two polypeptides of 15 and 30 kDa molecular weight. A 24 kDa polypeptide that was detected as
a marked band in extracts of primary callus was not detected in stages 1, 2, and 3. Further, the tissue level of a 50 kDa
glycoprotein decreased during transition from stage 2 to stage 3. However, the levels of glycoproteins in the medium were
markedly higher in stage 0 cultures compared to those in stage 1. The activities of a protein kinase, glycosidase, and xylanase
increased markedly with progressing embryogenesis. Our observations suggest that in addition to being controlled at the level
of stage-specific gene expression, somatic embryogenesis in sandalwood is also regulated at the level of controls on cell
wall flexibility and posttranslational changes in the pool of preexisting proteins. |
| |
Keywords: | somatic embryogenesis proteins protein kinase glycosidase xylanase sandalwood |
本文献已被 SpringerLink 等数据库收录! |
|