Role of NAADP for calcium signaling in the salivary gland |
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| Affiliation: | 1. Division of Immunology, Children''s Hospital, Boston, Mass;2. Department of Pediatrics, Harvard Medical School, Boston, Mass;3. Department of Molecular Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Mass;4. Department of Pediatrics, United Arab Emirates University, Al-Ain, United Arab Emirates;5. Division of Hematology-Oncology, Boston Children''s Hospital, Boston, Mass;6. Department of Pediatric Oncology, Dana-Farber Cancer Institute, Boston, Mass;7. Department of Pathology, Langone Medical Center, New York University, New York, NY;1. Key Laboratory of Glycoconjugate Research, Ministery Of Health, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Shanghai Medical College of Fudan University, Shanghai, China;2. Department of Urology, Zhongshan Hospital, Shanghai Medical College of Fudan University, Shanghai, China;3. Department of Urology, Ninth People׳s Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China;4. Department of Immunology, School of Basic Medical Sciences, Shanghai Medical College of Fudan University, Shanghai, China;1. Department of Dental Hygiene, College at Niigata, The Nippon Dental University, 1-8 Hamaura-cho, Chuo-ku, Niigata 951-8580, Japan;2. Department of Biochemistry, School of Life Dentistry at Niigata, The Nippon Dental University, 1-8 Hamaura-cho, Chuo-ku, Niigata 951-8580, Japan;3. Department of Histology, School of Life Dentistry at Niigata, The Nippon Dental University, 1-8 Hamaura-cho, Chuo-ku, Niigata 951-8580, Japan;4. Advanced Research Center, School of Life Dentistry at Niigata, The Nippon Dental University, 1-8 Hamaura-cho, Chuo-ku, Niigata 951-8580, Japan |
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| Abstract: | Coordination of intracellular Ca2+ signaling in parotid acini is crucial for controlling the secretion of primary saliva. Previous work from our lab has demonstrated acidic-organelle Ca2+ release as a participant in agonist-evoked signaling dynamics of the parotid acinar cell. Furthermore, results implicated a potential role for the potent Ca2+ releasing second messenger NAADP in these events. The current study interrogated a direct role of NAADP for Ca2+ signaling in the parotid salivary gland acinar cell. Use of live-cell Ca2+ imaging, patch-clamp methods, and confocal microscopy revealed for the first time NAADP can evoke or enhance Ca2+ dynamics in parotid acini. These results were compared with pancreatic acini, a morphologically similar cell type previously shown to display NAADP-dependent Ca2+ signals. Findings presented here may be relevant in establishing new therapeutic targets for those suffering from xerostomia produced by hypofunctioning salivary glands. |
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| Keywords: | Calcium signaling NAADP Parotid acinar cells Pancreatic acinar cells NED19 |
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