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果糖修饰壳聚糖微载体的制备及其原代大鼠肝细胞培养
引用本文:张立国,潘继伦,李结良,俞耀庭.果糖修饰壳聚糖微载体的制备及其原代大鼠肝细胞培养[J].生物工程学报,2003,19(1):116-119.
作者姓名:张立国  潘继伦  李结良  俞耀庭
作者单位:南开大学分子生物学研究所生物活性材料教育部重点实验室,天津,300071
基金项目:国家自然科学基金资助项目 (No .3 0 0 70 2 2 2 )~~
摘    要:利用果糖修饰的壳聚糖为材料,液体石蜡作分散介质,戊二醛作交联剂,通过反相悬浮法制备了性能优良的微米级果糖修饰壳聚糖微载体。用其进行原代大鼠肝细胞培养,利用相差显微镜和扫描电镜对细胞形态进行观察,并测定了细胞的代谢活性。结果显示果糖修饰的壳聚糖微载体是一种优良的肝细胞培养支架。

关 键 词:果糖,壳聚糖,微载体,肝细胞培养
文章编号:1000-3061(2003)01-0116-04
修稿时间:2002年6月26日

The Preparation of Fructose-modified Chitosan Microcarrier and Culture of Primary Rat Hepatocyte
ZHANG Li Guo PAN Ji Lun,LI Jie Liang YU Yao Ting.The Preparation of Fructose-modified Chitosan Microcarrier and Culture of Primary Rat Hepatocyte[J].Chinese Journal of Biotechnology,2003,19(1):116-119.
Authors:ZHANG Li Guo PAN Ji Lun  LI Jie Liang YU Yao Ting
Institution:The Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin 300071, China.
Abstract:Crosslinked chitosan microcarries were prepared by the reaction of glutaraldehyde with fructose-modified chitosan. Various factors that influence the preparation were studied and the reaction conditions were optimized. Morphology of rat hepatocyte cultured on chitosan microcarriers was observed using phase contrast microscopy and scanning electron microscope, the metabolic activity was measured. Rat hepatocytes cultured on chitosan microcarrier retained the spherical shape as they have in vivo and had high metabolic activity. Fructose can enhance the metabolic activity of hepatocytes and fructose-modified chitosan microcarrier is a promising scaffold for hepatocytes attachment, which can be used in bioartificial liver support system.
Keywords:fructose    chitosan    microcarrier    hepatocyte culture
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