Phagemid VPCS vectors for priming, cloning and sequencing |
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| Authors: | S A Krawetz D Sellos N C Wong G H Dixon |
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| Institution: | Department of Medical Biochemistry, Faculty of Medicine, University of Calgary, Alberta, Canada. |
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| Abstract: | A phagemid was adapted for use as the vector in the vector-primer-cloner-sequencer cloning system. The use of this new vector markedly expanded the utility of this technology for the construction of cDNA libraries. Technological advantages and new capabilities include: (1) a greater number of unique restriction sites within the polylinker region; (2) the ability to produce single-stranded templates for nucleotide sequencing, and (3) a convenient means to synthesize strand-specific hybridization probes. With the use of this cloning system, a rat liver cDNA library (8.56 x 10(5) recombinants from 1 microgram of poly(A)+ RNA) was rapidly (in two days) constructed. |
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