Circular Dichroism Studies of the Interaction of Tat Analogues Substituted in the Arg<sup>52</sup> Position with TAR RNA HIV-1期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Circular Dichroism Studies of the Interaction of Tat Analogues Substituted in the Arg⁵² Position with TAR RNA HIV-1

Authors:	Piotr Mucha Piotr Rekowski Agnieszka Szyk Gotfryd Kupryszewski Magorzata Giel-Pietraszuk Jan Barciszewski

Affiliation:	(1) Department of Chemistry, University of Gdask, Sobieskiego 18, PL-80-952 Gdask, Poland;(2) Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12, PL-61-704 Pozna, Poland

Abstract:	The Tat wild-type fragment of sequence Arg⁴⁹-Lys-Lys-Arg⁵²-Arg-Gln-Arg-Arg-Arg⁵⁷-NH₂ (labelled as Tat1) and three analogues of this fragment with the substitution Arg⁵² D-Arg⁵² (labelled as Tat2) or L-citrulline (Cit) (labelled as Tat3) or L-ornithine (Orn) (labelled as Tat4) were synthesized to study Tat-TAR RNA HIV-1 (27-nucleotide fragment of sequence 5-AGAUCUGAGCCUGGAGCUCUCU-3) interactions by circular dichroism. -helical structure was the most readily adopted by the Tat3 analogue with Arg⁵² Cit substitution. All the peptides investigated caused conformational changes in the TAR structure. The most dramatic changes were observed for the Tat2-TAR complex.

Keywords:	circular dichroism HIV Tat peptides Tat-TARinteraction
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