Enzymatic conversion of dihydrouracil into uracil |
| |
| Affiliation: | 1. Institut für Pharmazeutische und Medizinische Chemie der Universität Münster, Corrensstraße 48, D-48149 Münster, Germany;2. Department für Pharmazie, Ludwig-Maximilians-Universität München, Butenandtstr. 5-13, D-81377 München, Germany;3. Cells-in-Motion Cluster of Excellence (EXC 1003 – CiM), Westfälische Wilhelms-Universität Münster, Germany;1. Department of Chemistry, Lomonosov Moscow State University, 1 Leninskie Gory, Moscow 119991, Russian Federation;2. Institute of Physiologically Active Compounds, Russian Academy of Sciences, 1 Severny proezd, Chernogolovka, Moscow Region 142432, Russian Federation;1. Luxembourg Institute of Science and Technology (LIST), Materials Research and Technology Department (MRT), 5 rue Bommel, 4940 Hautcharage, Luxembourg;2. Université de Lorraine, LMOPS E.A. 4423, 57070 Metz, France;3. C2MA, Ecole des mines d''Alès, 6 avenue de Clavières, 30319 Alès, France |
| |
| Abstract: | We found that some strains of Rhodotorula glutinis can oxideze dihydrourcil to uracil, and we converted dihydrouracil into uracil using the resting and immobilized cells of R. glutinis IFO-1389.The optimum pH of the conversion of dihydrouracil into uracil was 7.8. Oxygen supply was essential to the conversion. With resting cells, the addition of both o-phenanthroline and Triton X-100 caused increase of the yield of uracil about ten times as much as that with no addition.The conversion ratios of dihydrouracil into uracil using immobilized-cell beds, which were made with chitosan and glutaraldehyde, were 100, 98, and 77% when the concentration of dihydrouracil were 1, 2, and 3 (w/v)%, respectively, for 68 h at 30°C. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
