Simultaneous saccharification and ethanol fermentation of sago starch using immobilized Zymomonas mobilis |
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| Affiliation: | 1. Department of Chemical and Petroleum Engineering, College of Engineering, Afe Babalola University, Ado-Ekiti, Nigeria;2. Department of Petroleum and Gas Engineering, Baze University, Abuja, Nigeria;3. Department of Mechanical and Mechatronics Engineering, College of Engineering, Afe Babalola University, Ado-Ekiti, Nigeria;4. Department of Civil Engineering, College of Engineering, Afe Babalola University, Ado-Ekiti, Nigeria |
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| Abstract: | Simultaneous saccharification and ethanol fermentation (SSF) of sago starch using amyloglucosidase (AMG) and immobilized Zymomonas mobilis ZM4 on sodium alginate was studied. The immobilized Zymomonas cells were more thermo-stable than free Zymomonas cells in this system. The optimum temperature in the SSF system was 40°C, and 0.5% (v/w) AMG concentration was adopted for the economical operation of the system. The final ethanol concentration obtained was 68.3 g/l and the ethanol yield, Yp/s, was 0.49 g/g (96% of the theoretical yield). After 6 cycles of reuse at 40°C with 15% sago starch hydrolysate, the immobilized Z. mobilis retained about 50% of its ethanol fermenting ability. |
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