Effect of temperature on the ultrastructure of bovine oocytes in culture |
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| Affiliation: | 1. Institute for Cattle Breeding and Dairy Science, Warsaw Agricultural University, 05–840 Brwinow, Poland;2. Institute for Zootechnics, Department of Animal Reproduction and Artificial Insemination, 32–083 Balice/Kraków, Poland;1. Research Group in Immunobiology and Pathogenesis, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Altos of Santa Helena, A.A 546, Ibagué, Colombia;2. Poultry Research Group, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Altos of Santa Helena, A.A 546, Ibagué, Colombia;1. School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, New South Wales, Australia;2. Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, Faculty of Science, University of Newcastle, Callaghan, New South Wales, Australia;3. MP Fertility, Central Coast, New South Wales, Australia |
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| Abstract: | Cattle oocytes were cultured at 35, 37 and 39°C to determine the effect of temperature on the maturation and degeneration of the cells, as indicated by changes in the ultrastructure of the cytoplasm. The culture temperature influences the characteristics of oocyte peripheral cytoplasm. A temperature of 35°C was most favourable, as indicated by the highest frequency of granulated vesicles. Growth rate of oocytes, measured by width of the perivitelline space and the distribution of cortical granules, is a little slower at 35° C than at 37 or 39° C. However, myelin bodies are less numerous at 35°C, which seems to indicate that this temperature is more favourable than higher temperatures. Higher frequency of myelin bodies could indicate a more advanced stage of metabolism or quicker degenerative changes in the cells. |
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