Some morphological and biochemical peculiarities of Datura innoxia callus- and regenerated cultures |
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Authors: | Natasha Missaleva Gizella Petri Éva Szõke |
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Institution: | (1) Institute of Pharmacognosy, Semmelweis University of Medicine, Üllõi str.26, H-1085 Budapest, Hungary |
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Abstract: | Five nutrient media were tested for expression of morphological pathway in Datura innoxia callus cultures. Regenerants-rooted plants and non-rooted shoots obtained from three subsequent subcultures of callus cultures were assayed for tropane alkaloid content and composition. Although no wide variation of bio-chemical traits of regenerants was observed. For a given number of plants decrease of hyoscyamine in their roots was detected. Alkaloid content and composition of upper parts of plants having roots and shoots or lacking roots was studied-receiving an idea about biosynthesis of these organs. |
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Keywords: | Datura innoxia meristematic structures organogenesis rooted regenerants shoot cultures tropane alkaloids |
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