Genetic and morphological characterization of an Escherichia coli chromosome segregation mutant. |
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Authors: | P S Stewart and R D''''Ari |
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Affiliation: | Institut Jacques Monod, Centre National de la Recherche Scientifique, Université Paris 7, France. |
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Abstract: | The temperature-sensitive nucleoid segregation mutant of Escherichia coli, PAT32, formerly described as a parA mutant, has been shown to carry a mutation near 66 min on the genetic map. Fine mapping with phages from the collection of Kohara et al. is consistent with its being a parC allele. Observation by fluorescence microscopy revealed the formation, at a nonpermissive temperature, of filaments containing one or two large nucleoids and of normal-size anucleate cells. There was also a significant loss of viability. |
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