多重PCR同时检测人乳头瘤病毒、巨细胞病毒和沙眼衣原体

为了应用聚合酶链反应同时检测人巨细胞病毒（CMV）、人乳头瘤病毒（HPV）、沙眼衣原体（CT），参照文献报道的基因序列，设计合成了三对能扩增370bp、450bp、510bp基因片段的引物，并对PCR扩增条件进行了优化。0.1fgHPV－DNA、CMV－DNA、CT－DNA即可被检出，得到了与设计片段相同的产物，且不扩增大肠杆菌、白色念球菌、解尿支原体等病原的核酸。对395例标本进行检查，各病原体的检出率分别是：HPV19.2%、CMV14.9%、CT5.1%。其中混合感染42例。PCR同时检测CMV、HPV、CT经济、快速、敏感、特异，可用于临床诊断和实验研究。

Simultaneous Detection of Cytomegalovirus,Human Papillomavirus and Chlamydozoa Trachomatis by Multiplex PCR

In order to develop a polymerase chain reaction-based assay for the simultaneous detection of cytomegalovirus(CMV), human papillomavirus(HPV) and chlamydozodtra chomatis (CT) from a single genital swab, we chose three sets of oligonucleotide primers according to CMV,HPV and CT gene sequences reported that amplified products of 370bp, 450bp, and 510bp. The conditions for multiplex PCR were optimized. By this method, 0.01fg of HPV-DNA,CMV-DNA and CT-DNA could be detected and visualized under UV light, but not the gene fragments of human lymphocyte-DNA, E.coli-DNA, U. urealyticum-DNA and C. albicans-DNA.395 clinical samples were tested, the positive rates for HPV, CMV and CT were 19.2%, 14.9% and 5.1% respectively, and besides, in 42 samples, these three agents mixedly infected. The multiplex PCR appears to be a rapid, sensitive and specific method for detecting CMV, HPV and CT simultaneouly from a single genital swab and may be applied to clinical diagnosis and laboratory works.