Cell reassociation behavior and lectin-induced agglutination of embryonic mouse cells from different brain regions |
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Authors: | M E Hatten R L Sidman |
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Institution: | 1. Department of Neuropathology, Harvard Medical School, Boston, MA 02115 USA;2. Department of Neuroscience, Children''s Hospital Medical Center, Boston, MA 02115, USA |
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Abstract: | Cells from dissociated embryonic mouse cerebellum, when plated at high cell density in a 10 μl microwell, assembled into a reproducible three-dimensional pattern of reaggregates containing 2–10000 cells each and interconnecting cables of processes. The details of this pattern were modifiable by coating the culture substratum with poly-d-lysine and by the use of different types of serum in the growth medium. Formation of interconnecting cables was inhibited reversibly by the dimeric lectin Succinyl Concanavalin A (Succ-ConA). Cells from cerebellum, midbrain, medulla and cerebral cortex, cultured separately in microwells, formed distinguishable patterns that appeared to be more region-specific than developmental stage-specific.With lectins as probes of carbohydrate-containing surface macromolecules, cells harvested from different tissue regions had different cell surface properties. In addition the concentration of concanavalin A (ConA) or wheat germ agglutinin (WGA) required for half maximal agglutination was greatly increased for cerebellar cells harvested later than embryonic day 16. These observations suggest both regional and age-specific alterations in cell surface elements required for lectin-induced agglutination during mouse brain development. |
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