Bright oligothiophene N-succinimidyl esters for efficient fluorescent labeling of proteins and oligonucleotides |
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Authors: | Barbarella Giovanna Zambianchi Massimo Ventola Alfredo Fabiano Eduardo Della Sala Fabio Gigli Giuseppe Anni Marco Bolognesi Andrea Polito Letizia Naldi Marina Capobianco Massimo |
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Affiliation: | Consiglio Nazionale Ricerche (ISOF), Mediteknology srl, Area Ricerca CNR, Via Gobetti 101, I-40129 Bologna, Italy. barbarella@isof.cnr.it |
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Abstract: | The synthesis of multicolor fluorescent oligothiophene N-succinimidyl esters (TSEs) is reported, and their optical properties are discussed with the aid of ab initio calculations. The esters were coupled to proteins and to 3'-amino-modified oligonucleotides in mild conditions and with similar modalities. A comparative study of the bioconjugate of IgG1 anti-CD3 antibody labeled with a blue fluorescent TSE and with fluorescein isothiocyanate (FITC) is reported, showing that the former achieves higher photoluminescence intensity and optical stability than the latter. Fluorescence resonance energy transfer experiments with TSE-labeled oligonucleotides and examples of cellular imaging via TSE-labeled proteins are reported. |
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