RNA Isolation method for polysaccharide rich algae: agar producing <Emphasis Type="Italic">Gracilaria tenuistipitata</Emphasis> (Rhodophyta) |
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Authors: | Vanessa Dos Reis Falcão Angela Pedroso Tonon Mariana Cabral Oliveira Pio Colepicolo |
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Institution: | 1.Departamento de Bioquímica,Universidade de S?o Paulo, Instituto de Química,S?o Paulo,Brazil;2.Departamento de Botanica,Universidade de S?o Paulo, Instituto de Biociências,S?o Paulo,Brazil;3.Departamento de Bioquímica,IQUSP,S?o Paulo,Brazil |
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Abstract: | RNA isolation is essential to study gene expression at the molecular level. However, RNA isolation is difficult in organisms
(plants and algae) that contain large amounts of polysaccharides, which co-precipitate with RNA. Currently, there is no commercial
kit available, specifically for the isolation of high-quality RNA from these organisms. Furthermore, because of the large
amounts of polysaccharides, the common protocols for RNA isolation usually result in poor yields when applied to algae. Here
we describe a simple method for RNA isolation from the marine red macroalga Gracilaria tenuistipitata var. liui Zhang et Xia (Rhodophyta), which can be applied to other plants and algae. |
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Keywords: | Gracilaria tenuistipitata Polysaccharides RNA extraction |
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