| 染色质构象捕获及其衍生技术 |
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| 引用本文： | 翟侃,武治印,于典科.染色质构象捕获及其衍生技术[J].生物化学与生物物理进展,2010,37(9):939-944. |
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| 作者姓名： | 翟侃 武治印 于典科 |
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| 作者单位： | 中国医学科学院肿瘤医院肿瘤研究所病因及癌变研究室，北京 100021;中国生物技术发展中心，北京 100036;中国医学科学院肿瘤医院肿瘤研究所病因及癌变研究室，北京 100021 |
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| 摘 要： | 染色质的构象在基因表达调节方面起重要作用．介绍了染色质构象捕获、环状染色质构象捕获、3C碳拷贝、ChIP-loop、ChIA-PET和Hi-C等技术的基本原理及发展历程，对影响实验结果准确性的主要因素进行了分析．目的是为在三维层面研究基因的表达调控介绍新的研究手段，为功能研究提供新思路，也为相关技术的应用提供理论参考．
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| 关 键 词： | 染色质空间构象，染色质构象捕获，基因表达调控，染色质相互作用 |
| 收稿时间： | 2010/3/29 0:00:00 |
| 修稿时间： | 6/3/2010 12:00:00 AM |
Chromosome Conformation Capture (3C) and 3C-based Methods |
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| ZHAI Kan,WU Zhi-Yin and YU Dian-Ke.Chromosome Conformation Capture (3C) and 3C-based Methods[J].Progress In Biochemistry and Biophysics,2010,37(9):939-944. |
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| Authors: | ZHAI Kan WU Zhi-Yin and YU Dian-Ke |
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| Institution: | Department of Etiology & Carcinogenesis, Cancer Institute and Hospital, Chinese Academy of Medical Sciences, Beijing 100021, China;China National Center for Biotechnology Development, Beijing 100036, China;Department of Etiology & Carcinogenesis, Cancer Institute and Hospital, Chinese Academy of Medical Sciences, Beijing 100021, China |
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| Abstract: | Chromosome conformation is thought to play an important role in gene expression regulation. Chromosome conformation capture (3C), circular chromosome conformation capture (4C), 3C-carbon copy (5C), ChIP-loop assay, ChIA-PET and Hi-C method were developed to study spatial organization of long genomic regions in living cells. All steps of 3C, those 3C-based methods and key procedures in experiment were also discussed. The aim of this paper is to introduce some new methods to study gene regulation in 3D. |
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| Keywords: | chromosome conformation chromosome conformation capture gene regulation chromatin interaction |
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