| 抑瘤基因NGX6启动子的克隆与功能鉴定 |
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| 引用本文： | 刘敏姬,王晓艳,沈守荣,李楠,张德才,彭娅,郭勤,李桂源. 抑瘤基因NGX6启动子的克隆与功能鉴定[J]. 生物化学与生物物理进展, 2010, 37(10): 1082-1089 |
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| 作者姓名： | 刘敏姬 王晓艳 沈守荣 李楠 张德才 彭娅 郭勤 李桂源 |
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| 作者单位： | 中南大学湘雅三医院消化内科，长沙 410013;中南大学湘雅三医院消化内科，长沙 410013;中南大学湘雅三医院消化内科，长沙 410013;中南大学湘雅三医院消化内科，长沙 410013;中南大学湘雅三医院消化内科，长沙 410013;中南大学湘雅三医院消化内科，长沙 410013;中南大学湘雅三医院消化内科，长沙 410013;中南大学湘雅医学院肿瘤研究所，长沙 410078 |
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| 基金项目： | 国家重点基础研究发展计划(973)(2006CB910503), 国家自然科学基金(30770972), 湖南省自然科学基金项目(09JJ3066, 07JJ3075)和中南大学研究生学位论文创新工程(2009bsxt042)资助项目 |
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| 摘 要： | NGX6是一个结直肠癌候选抑瘤基因，其转录调控机制不明．采用生物信息学技术预测其启动子区，并构建NGX6启动子荧光素酶报告基因重组体pGL3/Enhancer/1126．荧光素酶活性检测结果表明该区域具有强启动子活性．应用PromoterInspector program，FistEF，CpGplot和MatInspector Professional软件分析发现，NGX6基因转录调控区为一个不含TATA盒，而含有CAAT盒的GC富集区．凝胶迁移阻滞实验确定NGX6基因启动子区域具有Sp1特异性结合位点，Sp1特异性阻断剂光神霉素(mithramycin A)能明显抑制NGX6启动子的活性和NGX6基因的表达；封闭内源性Sp1能下调NGX6基因mRNA表达水平．
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| 关 键 词： | NGX6，转录调控，启动子，Sp1 |
| 收稿时间： | 2010-03-06 |
| 修稿时间： | 2010-07-14 |
Cloning and Identification of Promoter of Suppressed-tumor Gene NGX6 |
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| LIU Min-Ji,WANG Xiao-Yan,SHEN Shou-Rong,LI Nan,ZHANG De-Cai,PENG Y,GUO Qin and LI Gui-Yuan. Cloning and Identification of Promoter of Suppressed-tumor Gene NGX6[J]. Progress In Biochemistry and Biophysics, 2010, 37(10): 1082-1089 |
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| Authors: | LIU Min-Ji WANG Xiao-Yan SHEN Shou-Rong LI Nan ZHANG De-Cai PENG Y GUO Qin LI Gui-Yuan |
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| Affiliation: | Department of Gastroenterology, The Third Xiangya Hospital, Central South University, Changsha 410013, China;Department of Gastroenterology, The Third Xiangya Hospital, Central South University, Changsha 410013, China;Department of Gastroenterology, The Third Xiangya Hospital, Central South University, Changsha 410013, China;Department of Gastroenterology, The Third Xiangya Hospital, Central South University, Changsha 410013, China;Department of Gastroenterology, The Third Xiangya Hospital, Central South University, Changsha 410013, China;Department of Gastroenterology, The Third Xiangya Hospital, Central South University, Changsha 410013, China;Department of Gastroenterology, The Third Xiangya Hospital, Central South University, Changsha 410013, China;Cancer Research Institute, Xiangya Medical School of Central South University, Changsha 410078, China |
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