OKB7, a monoclonal antibody that reacts at or near the C3d binding site of human CR2 |
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| Authors: | P E Rao S D Wright E F Westberg G Goldstein |
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| Affiliation: | 1. Dept. of Immunology, Erasmus MC, University Medical Center Rotterdam, Wytemaweg 80 3015 CN, Rotterdam, The Netherlands;2. Dept. of Laboratory Medicine, Medical Immunology, University of Groningen, University Medical Center Groningen, Hanzeplein 1, 9700 RB, Groningen, The Netherlands;3. Dept. of Pediatrics, Beatrix Children''s Hospital, University of Groningen, University Medical Centre Groningen, Hanzeplein 1,9700 RB, Groningen, The Netherlands |
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| Abstract: | OKB7, an IgG2 mouse monoclonal antibody, binds to the C3d receptor (CR2) of human B lymphocytes in or near the active site of the receptor. OKB7 precipitates the same molecule as monoclonal antibodies B2 and HB-5 which have previously been reported to react with the C3d receptor at epitopes distant from the active site. The epitope recognized by OKB7 is distinct from those bound by B2 and HB-5 as shown in competitive binding experiments and is near the complement binding site since OKB7 blocks EAC3d rosetting at concentrations as low as 1 microgram/ml in the absence of cross-linking antibodies. |
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