解磷菌PSB-R全基因组测序鉴定及其解磷特性分析

采用生理生化指标结合分子生物学技术对解磷菌PSB-R进行分类学鉴定，确定为粘质沙雷氏菌（Serratia marcescens），通过二代测序平台Illumina NovaSeq PE150对PSB-R进行全基因组测序，分析预测了与解磷能力相关基因及其他植物促生基因组成情况。通过响应面优化试验检测了PSB-R最大解磷能力为805.199 mg/L，连续培养10代后解磷能力稳定且对多种难溶性磷酸盐均具有溶解能力。本研究为解磷菌解磷机制的进一步研究提供了基因组数据基础，同时证实PSB-R具有应用于菌肥的潜力，为后续解磷菌肥的研制提供了研究基础。

Whole-Genome Sequencing Identification of Phosphate-solubilizing Bacteria PSB-R and Analysis of Its Phosphate-solubilizing Properties

The phosphate-solubilizing bacterium PSB-R was identified as Serratia marcescens by physiological and biochemical indicators combined with molecular biology technology. Second-generation sequencing platform Illumina NovaSeq PE150 was used to have the whole genome of PSB-R sequenced, and to analyze and predict the composition of genes related to phosphate-solubilizing ability and other plant growth-promoting genes. The response surface optimization test showed that the maximum phosphorus dissolving capacity of PSB-R was 805.199 mg/L. After 10 generations of continuous culture, the phosphorus dissolving capacity was stable and had the ability to dissolve a variety of insoluble phosphates. This study provides a genomic data basis for the further study of the mechanism of phosphate solubilization by phosphate solubilizing bacteria, and confirms that there is a potential for PSB-R to be applied to bacterial fertilizer, which provides a research basis for the subsequent development of phosphate-solubilizing bacterial fertilizer.