Significance of Urinary Full-Length Megalin in Patients with IgA Nephropathy |
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| Authors: | Takuto Seki Katsuhiko Asanuma Rin Asao Kanae Nonaka Yu Sasaki Juan Alejandro Oliva Trejo Hiroyuki Kurosawa Yoshiaki Hirayama Satoshi Horikoshi Yasuhiko Tomino Akihiko Saito |
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| Affiliation: | 1. Division of Nephrology, Juntendo University Faculty of Medicine, Tokyo, Japan.; 2. TMK project, Medical Innovation Center, Kyoto University Graduate School of Medicine, Kyoto, Japan.; 3. Reagents Development Department, Denka Seiken Co. Ltd., Niigata, Japan.; 4. Department of Applied Molecular Medicine, Niigata University Graduate School of Medicine and Dental Sciences, Niigata, Japan.; University of Bari Aldo Moro, Italy, |
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| Abstract: | Background and ObjectivesMegalin is highly expressed at the apical membranes of proximal tubular epithelial cells. A urinary full-length megalin (C-megalin) assay is linked to the severity of diabetic nephropathy in type 2 diabetes. This study examined the relationship between levels of urinary C-megalin and histological findings in adult patients with IgA nephropathy (IgAN).Design, Setting, Participants, & MeasurementsUrine samples voided in the morning on the day of renal biopsy were obtained from 73 patients with IgAN (29 men and 44 women; mean age, 33 years) and 5 patients with membranous nephropathy (MN). Renal pathologic variables were analyzed using the Oxford classification of IgAN, the Shigematsu classification and the Clinical Guidelines of IgAN in Japan. The levels of urinary C-megalin were measured by sandwich ELISA.ResultsHistological analysis based on the Oxford classification revealed that the levels of urinary C-megalin were correlated with mesangial hypercellularity in IgAN patients (OR = 1.76, 95% CI: 1.04–3.27, P<0.05). There was a significant correlation between the levels of urinary C-megalin and the severity of chronic extracapillary abnormalities according to the Shigematsu classification in IgAN patients (β = 0.33, P = 0.008). The levels of urinary C-megalin were significantly higher in all risk levels of IgAN patients requiring dialysis using the Clinical Guidelines of IgAN in Japan than in the control group. The levels of urinary C-megalin were significantly higher in the high risk and very high risk grades than in the low risk grade (P<0.05). The levels of urinary C-megalin were significantly higher in MN patients compared to the control group.ConclusionsThe levels of urinary C-megalin are associated with histological abnormalities in adult IgAN patients. There is a possibility that urinary C-megalin is an independent predictor of disease progression of IgAN. In addition, our results suggest that urinary C-megalin is a marker of glomerular abnormalities in various glomerular diseases as well as IgAN. |
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