14C2H4: Distribution of 14C-labeled tissue metabolites in pea seedlings |
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Authors: | Giaquinta, Robert Beyer, Elmo, Jr. |
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Affiliation: | Central Research and Development Department Experimental Station E. I. du Pont de Nemours and Company Wilmington, Delaware 19898, U.S.A. |
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Abstract: | The 14C-metabolite distribution pattern following 14C2H4 metabolismin intact pea seedlings (Pisum sativum L.) was determined undervarious conditions. After a 24 hr exposure to 14C2H4, the majorityof 14C-metabolites were water-soluble (6070%) with lesseramounts in the protein (1015%), lipid (1%), and insoluble(12%) fractions. Ion exchange chromatography of the water-solublecomponents into basic, neutral, and acidic fractions revealeda 50 : 40 : 10 distribution, respectively. Chromatography ofthe neutral fraction revealed two regions of radioactivity (Rf=0.38)and 0.63 which did not cochromatograph with twenty-two knownsugars or neutral metabolites. Chromatograms of the basic fractioncontained 3 regions of radioactivity. Similar distribution patternswere noted when 14C2H4 exposure was followed by a 6 hr air chaseor when 5% CO2, an antagonist of ethylene action, was presentduring the exposure. Marked differences in the 14C-metabolite distribution patternswere obtained when 14CO2 was substituted for 14C2H4. These resultsindicate that the metabolic pathway involved in ethylene metabolismis different from that involved in intermediary carbon metabolism. 1 Contribution No. 2338 from Central Research and DevelopmentDepartment, Experimental Station, E. I. du Pont de Nemours andCompany, Wilmington, Delaware. (Received June 28, 1976; ) |
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