Distribution of Bm1, a SINE type transposable element, in cecropin B genes of the silkworm, Bombyx mori |
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Authors: | Kiyoko Taniai Minoru Yamakawa |
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Affiliation: | Laboratory of Biological Defense, National Institute of Sericultural and Entomological Science, Tsukuba, Ibaraki 305-8634, Japan |
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Abstract: | In order to investigate the distribution of Bm1, a SINE type transposable element, in cecropin B genes of the silkworm, Bombyx mori, a genomic library was first screened with cecropin B cDNA as a probe. Eighteen out of 275 positive clones were selected at random and SalI digestion patterns were compared. Ten clones which showed different patterns were further analyzed by Southern blotting using a cecropin B cDNA fragment encoding exon 1, exon 2 or the entire coding region as probes. The same SalI digested genomic clones were hybridized with a Bm1 probe. Comparison of positive patterns hybridized with the Bm1 probe to those hybridized with the cecropin B probes indicated that all cecropin B-fragments except one fragment had Bm1. The exceptional fragment contained exon 2 only. The results indicate that Bm1 is widely distributed in cecropin B genes. |
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Keywords: | Bm1 SINE Cecropin B gene Southern blotting Antibacterial peptide Insect immunity Bombyx mori |
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