Effect of isorhapontigenin on respiratory burst of rat neutrophils |
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Authors: | Ya. Nan Fang Geng Tao Liu |
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Affiliation: | aInstitute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical, College, Beijing, China |
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Abstract: | The effect of Isorhapontigenin (Iso) isolated from Belamcanda chinensis on respiratory burst of rat neutrophils was investigated. Iso (1, 10, 100 mmol/l) showed an inhibitory effect on superoxide anion and hydrogen peroxide production in phorbol myristate acetate (PMA) activated rat neutrophils in a concentration-dependent manner. Scanning electron microscopy detected that Iso (100 mmol/l) protected against surface changes in rat neutrophils stimulated with PMA. Also, 100 mmol/l Iso inhibited the release of beta-glucuronidase from the activated neutrophils. Electron-spin resonance (ESR) detected that Iso scavenged oxygen free radicals generated in the PMA activated Neutrophils. These results suggest that Iso inhibits respiratory burst of PMA-activated rat neutrophils by scavenging oxygen free radicals. |
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Keywords: | Isorhapontigenin neutrophil oxygen free radicals anti-oxidant β-glucuronidase |
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