Serotonin-immunoreactive neurones in the brain of Locusta migratoria innervating the corpus cardiacum |
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Authors: | P. N. M. Konings Dr. H. G. B. Vullings R. Siebinga J. H. B. Diederen W. F. Jansen |
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Affiliation: | (1) Research Group for Histology and Cell Biology of Regulatory Systems, Department of Experimental Zoology, University of Utrecht, Utrecht, The Netherlands;(2) Department of Experimental Zoology, Padualaan 8, NL-3584 CH Utrecht, The Netherlands |
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Abstract: | Summary The serotoninergic innervation of the corpus cardiacum (CC) of Locusta migratoria was investigated using two antisera against serotonin. A dense network of immunoreactive nerve fibres was present in the storage lobe of the CC. Immunopositive fibres only sporadically crossed the border between the storage lobe and the glandular lobe of the CC. Immunopositive fibres entered the storage lobe of the CC via the nervus corporis cardiaci I (NCCI); NCCII was immunonegative. Unilateral retrograde fillings of the NCCI with the fluorescent tracer Lucifer yellow, followed by antiserotonin immunocytochemistry, revealed about 20 double-labelled neurones in the anterior part of the pars intercerebralis. The double-labelled neurones were scattered between fluorescent non-immunoreactive neurones. Additionally, 5–7 neurones labelled only with Lucifer yellow were found at the ventrolateral side of the tritocerebrum. No immunopositive neurones were observed in the hypocerebral ganglion. Immunopositive fibres from neurones in the frontal ganglion ran via the recurrent nerve and the neuropile of the hypocerebral ganglion into the paired oesophageal nerve. At most, a few immunopositive nerve fibres occurred in the cardiostomatogastric nerves II, which connect the storage lobe of the CC with the paired oesophageal nerve at the caudal end of the hypocerebral ganglion. |
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Keywords: | Corpus cardiacum Serotonin Immunocytochemistry Lucifer yellow Double labelling Locusta migratoria (Insecta) |
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