Genetic and biochemical analysis of Hypericum perforatum L. plants regenerated after cryopreservation |
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Authors: | Martina Urbanová Ján Ko?uth Eva ?ellárová |
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Institution: | (1) Institute of Biology and Ecology, Faculty of Science, P. J. Šafárik University, Mánesova 23, 04154 Košice, Slovakia |
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Abstract: | Shoot-tips from in vitro cultured Hypericum perforatum L. genotypes were subjected to assessments of developmental competence, genetic stability, and biosynthetic ability to identify
critical points during cryopreservation. Survival rate, chromosome number stability, alteration in VNTR sequences and hypericin
content were evaluated, in plants after pre-culture, and two subsequent cryogenic steps (cryoprotection and cooling) and those
recovered from cryopreserved meristems. Pre-culture and cryoprotection treatments, did not reveal any significant differences,
in these studied characteristics. Genetic stability was assessed by chromosome counts and analysis of variability in the VNTR
sequences. No changes in chromosome number were detected in comparison with the untreated control but minor alterations were
revealed in non-coding sequences. The content of hypericin after the recovery of cryopreserved meristems remained comparable
with the unfrozen control. The controlled rate freezing technique used for cryopreservation was relevant for restoration of
genetic and biochemical stability in Hypericum perforatum L. shoot-tips. |
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Keywords: | Chromosome number Hypericin content Survival rate VNTR sequences |
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