Non-covalent enzyme-polyelectrolyte complexes as self-buffered catalysts for use in low-water organic media |
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| Authors: | Alexander V. Vakurov Alexander K. Gladilin Johann Partridge Vadim V. Mozhaev Andrey V. Levashov Peter J. Halling |
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| Affiliation: | (1) Division of Chemical Enzymology, Chemistry Department, M.V. Lomonosov Moscow State University, 119899 Moscow, Russia;(2) N.N. Semenov Institute of Chemical Physics, 117977 Moscow, Russia;(3) Department of Bioscience & Biotechnology, University of Strathclyde, G1 1XW Glasgow, UK |
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| Abstract: | Summary When free chymotrypsin is used to catalyse hydrolysis of N-acetyl tyrosine ethyl ester in 90% acetonitrile, the reaction rate soon falls because of the accumulation of the acidic product. If the enzyme is used in the form of a suspended complex with polyacrylic acid, the polyelectrolyte acts as an acid-base buffer to permit extended reaction. |
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