|
|||||
|
|
Functional requirement of CCN2 for intramembranous bone formation in embryonic mice |
|
| Authors: | Kawaki Harumi Kubota Satoshi Suzuki Akiko Yamada Tomohiro Matsumura Tatsushi Mandai Toshiko Yao Mayumi Maeda Takeyasu Lyons Karen M Takigawa Masaharu |
| Affiliation: | a Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8525, Japan b Division of Oral Anatomy, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan c Department of Oral and Maxillofacial Reconstructive Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan d Department of Oral and Maxillofacial Surgery and Biopathology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan e Department of Orthopaedic Surgery, UCLA School of Medicine, Los Angeles, CA, USA |
| Abstract: | CCN2 is best known as a promoter of chondrocyte differentiation among the CCN family members, and Ccn2 null mutant mice display skeletal dysmorphisms. However, little is known concerning the roles of CCN2 during bone formation. We herein present a comparative analysis of wild-type and Ccn2 null mice to investigate the roles of CCN2 in bone development. Multiple histochemical methods were employed to analyze the effects of CCN2 deletion in vivo, and effects of CCN2 on the osteogenic response were evaluated with the isolated and cultured osteoblasts. As a result, we found a drastic reduction of the osteoblastic phenotype in Ccn2 null mutants. Importantly, addition of exogenous CCN2 promoted every step of osteoblast differentiation and rescued the attenuated activities of the Ccn2 null osteoblasts. These results suggest that CCN2 is required not only for the regulation of cartilage and subsequent events, but also for the normal intramembranous bone development. |
| Keywords: | Connective tissue growth factor Null mutant Osteoblast Differentiation Intramembranous bone formation CCN family |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |