Minimization of a eukaryotic mini-intein |
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Authors: | Elleuche Skander Döring Kristin Pöggeler Stefanie |
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Institution: | Institute of Microbiology and Genetics, Department of Genetics of Eukaryotic Microorganisms, Georg-August-University of Göttingen, Grisebachstrasse 8, 37077 Gottingen, Germany |
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Abstract: | Inteins are internal protein splicing elements that can autocatalytically self-excise from their host protein and ligate the protein flanks (exteins) with a peptide bond. Large inteins comprise independent protein splicing and endonuclease domains whereas mini-inteins lack the central endonuclease domain. To identify mini-intein domains that are essential for protein splicing, deletions were introduced at different sites of the 157-aa PRP8 mini-intein of Penicillium chrysogenum. The removal of eight and six amino acids at two different sites resulted in a functional eukaryotic mini-intein of only 143 aa. |
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Keywords: | Intein Protein splicing Penicillium chrysogenum PRP8 protein Minimization |
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