Novel evidence suggesting Clostridium difficile is present in human gut microbiota more frequently than previously suspected |
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Authors: | Iizuka Masahiro Konno Shiho Itou Hiroaki Chihara Junichi Toyoshima Itaru Horie Yasuo Sasaki Kenji Sato Akiko Shindo Kenichi Watanabe Sumio |
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Institution: | Department of Internal Medicine, Akita University School of Medicine, Japan. |
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Abstract: | Prevalence rate of Clostridium difficile in healthy human adults is believed to be very low. Our RT-PCR system using glass powder, which can eliminate PCR inhibitors, detected C. difficile toxin B mRNA in 16 of 30 fecal samples (53.3%) from healthy human adults. In contrast, we failed to detect toxin B in the same fecal samples by PCR using DNA templates extracted with phenol-chloroform. Our results suggest that PCR inhibitors in feces carried through phenol-chloroform extraction procedure might suppress the sensitivity of PCR and that C. difficile is actually present in human gut microbiota more frequently than previously suspected. |
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Keywords: | clostridium difficile toxin B PCR healthy human adult |
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