Kinetic characterization of the glycosyltransferase module of Staphylococcus aureus PBP2 |
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| Authors: | Barrett Dianah Leimkuhler Catherine Chen Lan Walker Deborah Kahne Daniel Walker Suzanne |
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| Institution: | Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA. |
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| Abstract: | We report the heterologous overexpression and purification of Staphylococcus aureus PBP2 and demonstrate efficient glycan polymerization from lipid II in vitro. S. aureus PBP2 is the first purified gram-positive class A penicillin-binding protein to show good transglycosylase activity. This enables further studies on this important class of enzymes. |
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