Cytotoxicity of fungal metabolites to lepidopteran (Spodoptera frugiperda) cell line (SF-9) |
| |
Authors: | Fornelli Francesca Minervini Fiorenza Logrieco Antonio |
| |
Affiliation: | Istituto di Scienze delle Produzioni Alimentari, Consiglio Nazionale delle Ricerche, Viale Einaudi 51, 70125 Bari, Italy. francesca.fornelli@ispa.cnr.it |
| |
Abstract: | The toxicity of sixteen fungal metabolites produced by some entomopathogenic fungi or biological control fungi agents was evaluated on lepidopteran Spodoptera frugiperda (SF-9) cell line by Trypan blue dye exclusion and MTT-colorimetric assay, after 48 h of incubation. No statistical difference was found between IC50values (50% Inhibiting Concentration) and CC50 values (50% Cytotoxicity Concentration) obtained by MTT test and Trypan blue dye exclusion for each fungal metabolite. By MTT assay, the cytotoxicity ranking was fusarenon X (IC50 0.3 microM) = diacetoxyscirpenol (IC50 0.5 microM) = beauvericin (IC50 2.5 microM) = nivalenol (IC50 5.3 microM) = enniatin (IC50 6.6 microM) > or = gliotoxin (IC50 7.5 microM) > zearalenone (IC50 17.5 microM) > deoxynivalenol (IC50 47.6 microM). By Trypan blue dye exclusion the cytotoxicity ranking was fusarenon X (CC50 0.4 microM) = diacetoxyscirpenol (CC50 1.1 microM) beauvericin = (CC50 3.0 microM)=gliotoxin (CC50 4.0 microM) = enniatin (CC50 6.7 microM) > or = nivalenol (CC50 9.5 microM) > zearalenone (CC50 18.3 microM) > deoxynivalenol (CC50 45.0 microM). The comparison with other bioassays showed that the SF-9 insect cell line could represent a further tool to screen for the toxic effects of fungal metabolites especially for beauvericin, gliotoxin, and zearalenone. |
| |
Keywords: | Fungal metabolites Entomopathogenic fungi Spodoptera frugiperda cell line (SF-9) Cytotoxicity MTT-colorimetric assay Trypan blue dye exclusion |
本文献已被 ScienceDirect PubMed 等数据库收录! |