Characterization of the first eukaryotic cold-adapted patatin-like phospholipase from the psychrophilic Euplotes focardii: Identification of putative determinants of thermal-adaptation by comparison with the homologous protein from the mesophilic Euplotes crassus |
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Authors: | Guang Yang Concetta De Santi Donatella de Pascale Sandra Pucciarelli Stefania Pucciarelli Cristina Miceli |
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Institution: | 1. School of Biosciences and Biotechnology, University of Camerino, Italy;2. Institute of Protein Biochemistry, CNR, Naples, Italy |
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Abstract: | The ciliated protozoon Euplotes focardii, originally isolated from the coastal seawaters of Terra Nova Bay in Antarctica, shows a strictly psychrophilic phenotype, including optimal survival and multiplication rates at 4–5 °C. This characteristic makes E. focardii an ideal model species for identifying the molecular bases of cold adaptation in psychrophilic organisms, as well as a suitable source of novel cold-active enzymes for industrial applications. In the current study, we characterized the patatin-like phospholipase from E. focardii (EfPLP), and its enzymatic activity was compared to that of the homologous protein from the mesophilic congeneric species Euplotes crassus (EcPLP). Both EfPLP and EcPLP have consensus motifs conserved in other patatin-like phospholipases. |
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Keywords: | Patatin-like phosholipase Molecular cold adaptation Site-directed mutagenesis Psychrophilic enzymes |
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