Subcellular localization of acetycholinesterase molecular forms in endplate regions of adult mammalian skeletal muscle |
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Authors: | Hugo L Fernandez Nibaldo C Inestrosa Joel R Stiles |
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Institution: | (1) Neuroscience Research Laboratory, Veterans Administration Medical Center, 4801 Linwood Blvd., 64128 Kansas City, Missouri;(2) Department of Physiology, University of Kansas Medical Center, 66103 Kansas City, Kansas;(3) Department of Cell Biology, Catholic University, Santiago, Chile |
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Abstract: | The characterization of individual acetylcholinesterase (AChE) molecular form subcellular pools in adult mammalian skeletal muscle is a critical point when considering such questions as the origin, assembly, and neurotrophic regulation of these molecules. By correlating the results of differential extraction, in vitro collagenase digestion, and in situ pharmacologic probes of AChE molecular forms in endplate regions of adult rat anterior gracilis muscle, we have shown that: 1) 4.0S (G1) and 6.0S (G2) AChE are predominantly membrane-bound and intracellular; if an extracellular and/or soluble fraction of these forms exists, it cannot be adequately resolved by our methods; 2) 9–11S (globular) AChE activity is distributed between internal and external pools, as well as membrane-associated and soluble fractions; 3) 16.0S (A12) AChE is not an integral membrane protein and exists both intracellularly (25–30%) and extracellularly (70–75%). |
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