首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Overproduction and preliminary X-ray characterization of aspartate aminotransferase from Escherichia coli
Authors:S Kamitori  K Hirotsu  T Higuchi  K Kondo  K Inoue  S Kuramitsu  H Kagamiyama  Y Higuchi  N Yasuoka  M Kusunoki
Abstract:The aspartate aminotransferase of Escherichia coli was overproduced in cells after genetic manipulation, and was crystallized from a polyethylene glycol solution, pH 7.0. The crystals obtained were of good quality and had diffractions extending beyond 2.4 A. The space group and unit cell dimensions were determined with a precession camera and a four-circle diffractometer to be C222(1), and a = 157.1 A, b = 85.5 A, and c = 79.7 A, respectively. Only one protein subunit is contained in an asymmetric unit.
Keywords:
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号