Nucleolus and large nucleolar aggregates of condensed chromatin in interphase nuclei of L 929 cells |
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Authors: | Dr M Pouchelet A Anteunis A Gansmuller |
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Institution: | (1) Centre de Physiologie et d'Immunologie Cellulaires, INSERM U.104, C.N.R.S. et Association Claude Bernard, Hopital Saint Antoine, Paris, France;(2) INSERM U. 104 Hôpital St-Antoine, 75571 Paris Cedex 12, France |
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Abstract: | Summary Three-dimensional reconstructions show that the nucleoli from L 929 cells are associated with one or several large aggregates of chromatin displaying a honeycomb-like structure. The form and the number of both nucleoli and honeycomb structures vary as the cells emerge from the resting state and enter exponential growth. Quantitative data show that the number of honeycomb structures decreases as the number of nucleoli diminishes; both numerical regressions are significant. In addition, the nucleoli and the honeycomb structures enlarge when the cells enter the exponential growth phase.In resting cells the number of honeycomb structures is correlated to the number of nucleoli. Therefore we conclude that the large nucleolar mass of condensed chromatin, which in L 929 cells displays a honeycomb structure, contains a portion of the nucleolar organizing region. |
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Keywords: | Condensed chromatin Nucleolar organizing region Cell culture L929 cells |
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