Location and identity of the acyl substituents on the extracellular polysaccharides of Rhizobium trifolii and Rhizobium leguminosarum |
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| Institution: | 1. Qiushi Academy for Advanced Studies, Zhejiang University, China;2. College of Computer Science and Technology, Zhejiang University, China;3. School of Software Engineering, Tongji University, China;1. State Key Laboratory of Information Engineering in Surveying, Mapping and Remote Sensing, Wuhan University, 129 Luoyu Road, Wuhan 430079, China;2. School of Electronics Information and Communications, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China;3. Wuhan City Land Resources and Planning Information Center, 13 Sanyang Road, Wuhan 430014, China;4. Guangzhou Urban Planning and Design Survey Research Institute, 10 Jianshedamalu, Guangzhou 510060, China;1. State Key Laboratory of Petroleum Resources and Prospecting, National Engineering Laboratory for Offshore Oil Exploration, China University of Petroleum-Beijing, Beijing, 102249, China;2. State Key Laboratory of Marine Geology, School of Ocean and Earth Science, Institute for Advanced Study, Tongji University, Shanghai, 200092, China;1. Artificial Intelligence Key Laboratory of Sichuan Province, Sichuan Province University Key Laboratory of Bridge Nondestruction Detecting and Engineering Computing, Sichuan University of Science and Engineering, Zigong 643000, China;2. Department of Computing Science, University of Alberta, Edmonton, AB T6G 2H1, Canada;3. Department of Computing Science, Sichuan University, Chengdu 610065, China |
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| Abstract: | The basic structures of the extracellular polysaccharides of Rhizobium leguminosarum and Rhizobium trifolii were found to be identical, but their acylation patterns differ. Liquid hydrogen fluoride at −40° degrades the two polysaccharides to a series of oligosaccharides representing the repeating units of the polysaccharides and their higher homologs. At −23°, it degrades the polymers to a mixture of oligosaccharides from which a tetrasaccharide constituting a unit of the backbone of the polysaccharide, and a trisaccharide representing all but the non-reducing terminus of the side chain, could be readily purified. The location and identity of the acyl substituents were determined by 1H-n.m.r. spectroscopy, methylation analysis, and f.a.b. mass spectrometry. The unusual substituent d-3-hydroxybutanoate was found esterified to O-3 of a terminal 4,6-O-pyruvic acetalated d-galactose in both strains of R. leguminosarum, and in one of the three strains of R. trifolii tested. All of the strains tested contained a 3-O-acetyl substituent on the (1→4)-β-d-glucopyranosyl residues in the backbone of the polysaccharide. Only the R. leguminosarum polysaccharides contained a combination of 2- and 3-O-acetyl groups on the branching sugar of the backbone of the polymer. |
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