Spectroscopic studies of pig kidney diamine oxidase-anion complexes |
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| Institution: | 1. Department of Environmental Engineering, Norwegian Geotechnical Institute (NGI), P.O. Box 3930, Ullevål Stadion, NO-0806 Oslo, Norway;2. Department of Industrial Economics and Technology Management, Norwegian University of Technology, Trondheim, Norway;3. Ithaka Institute for Carbon Strategies, Ancienne Eglise 9, 1974 Arbaz, Switzerland;4. Faculty of Environmental Sciences and Natural Resource Management (MINA), Norwegian University of Life Sciences (NMBU), P.O. Box 5003, NO-1432 Ås, Norway;1. Department of Chemistry, Yasouj University, Yasouj, 75918-74831, Iran;2. Department of Chemistry, Isfahan University, Isfahan, 81746-73441, Iran;3. School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang, 212003, Jiangsu Province, China;4. Department of Environmental Toxicology, University of California, Davis, CA, 95616, USA;5. Department of Chemistry, National University of Singapore, 3 Science Drive 3, 117543, Singapore;1. Faculty of Chemical and Petroleum Engineering, University of Tabriz, Tabriz, Iran;2. Department of Medical Nanotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran;3. Research Centre for Pharmaceutical Nanotechnology and School of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran |
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| Abstract: | Complexes of pig kidney diamine oxidase with azide, thiocyanate, and cyanide have been characterized by EPR and circular dichroism spectroscopy. Cu(II) d-d bands are observed in the CD spectrum of the resting enzyme at ≈800 nm (12 500 cm−1) and 575 nm (17 400 cm−1). Anion binding produces characteristic changes in the CD spectra. N3−/SCN− → Cu(II) ligand-to-metal charge-transfer transitions are located at 390 nm (25 600 cm−1) and 365 nm (27 400 cm−1), respectively. In addition, an intense new band grew in at ≈500 nm (20 000 cm−1) when azide or thiocyanate were added, which may be assigned as a Cu(II) electronic transition that gains rotational strength in the anion complex. EPR spectra established that the Cu(II)-anion complexes are tetragonal; however, the magnitudes of the anion-induced shifts in the EPR parameters were consistent with substantial perturbations of the Cu(II) electronic ground state in the thiocyanate and cyanide complexes. Prominent superhyperfine splitting was apparent in the EPR spectra of the diamine oxidase complexes with thiocyanate and cyanide. The superhyperfine structure is (at least) partially attributable to endogenous Cu(II) ligands, probably from imidazole. |
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