A Meloidogyne graminicola C-type lectin,Mg01965, is secreted into the host apoplast to suppress plant defence and promote parasitism |
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| Authors: | Kan Zhuo Diana Naalden Silke Nowak Nguyen Xuan Huy Lander Bauters Godelieve Gheysen |
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| Affiliation: | 1. Laboratory of Plant Nematology, South China Agricultural University, Guangzhou, 510642 China;2. Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, 9000 Ghent, Belgium;3. Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, 9000 Ghent, Belgium Biology Department, College of Education, Hue University, 34 Le Loi, Hue, Vietnam |
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| Abstract: | C-type lectins (CTLs), a class of multifunctional proteins, are numerous in nematodes. One CTL gene, Mg01965, shown to be expressed in the subventral glands, especially in the second-stage juveniles of the root-knot nematode Meloidogyne graminicola, was further analysed in this study. In vitro RNA interference targeting Mg01965 in the preparasitic juveniles significantly reduced their ability to infect host plant roots. Immunolocalizations showed that Mg01965 is secreted by M. graminicola into the roots during the early parasitic stages and accumulates in the apoplast. Transient expression of Mg01965 in Nicotiana benthamiana and targeting it to the apoplast suppressed the burst of reactive oxygen species triggered by flg22. The CTL Mg01965 suppresses plant innate immunity in the host apoplast, promoting nematode parasitism in the early infection stages. |
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| Keywords: | apoplast C-type lectin effector Meloidogyne graminicola PTI suppression RNAi |
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