The in vitro production of an anthocyanin from callus cultures of Oxalis linearis |
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Authors: | H J Meyer J Van Staden |
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Institution: | (1) NU Research Unit For Plant Growth and Development, Department of Botany, University of Natal, 3200 Pietermaritzburg, Republic of South Africa |
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Abstract: | Callus growth and the production of anthocyanins were sustained on the salts and vitamins of Murashige and Skoog. Callus growth was stimulated at a concentration of 8–32 M -naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-d). Benzyladenine (BA) and zeatin at 8 M inhibited callus growth whereas isopentenyladenine (iP) stimulated callus growth. NAA repressed anthocyanin production with an increase in NAA from 8–32 M. Anthocyanin synthesis was promoted by an increase in 2,4-d from 0.5 to 2 M and decreased thereafter up to a concentration 32 M 2,4-d. A concentration of 8 M BA, thidiazuron and zeatin, respectively stimulated pigment production. Sucrose stimulated callus growth at 60 mM and pigment production at 120–360 mM.Abbreviations BA
6-benzyladenine
- 2,4-d
2,4-dichlorophenoxyacetic acid
- NAA
-naphthaleneacetic acid
- iP
isopentenyladenine
- TZ
thidiazuron-N-phenyl-N -1,2,3-thiadiazol-5-yl-urea
- Bu-HCl
Butanol-2N HCl
- BAW
Butanol-acetic acid-water |
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Keywords: | anthocyanins auxins callus culture cytokinins Oxalis linearis sucrose |
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