Optimization and modeling of phenanthrene degradation by <Emphasis Type="Italic">Mycobacterium</Emphasis> sp. 6PY1 in a biphasic medium using response-surface methodology |
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Authors: | Arwa Abdelhay Jean-Pierre Magnin Nicolas Gondrexon Stéphane Baup John Willison |
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Institution: | (1) Laboratoire d’Electrochimie at de Physico-chimie des Matériaux et des Interfaces (LEPMI), Institut National Polytechnique de Grenoble, ENSEEG, no 1130 rue de la Piscine, BP 75, Domaine Universitaire 38402 Saint-Martin d’Heres Cedex, France;(2) Laboratoire d’Electrochimie et de Physico-chimie des Matériaux et des Interfaces (LEPMI), Centre National de la Recherche Scientifique (CNRS), ENSEEG, no 1130 rue de la Piscine, BP 75, Domaine Universitaire 38402 Saint-Martin d’Heres Cedex, France;(3) Laboratoire d’Electrochimie et de Physico-chimie des Matériaux et des Interfaces (LEPMI), Université Joseph Fourier, ENSEEG, no 1130 rue de la Piscine, BP 75, Domaine Universitaire 38402 Saint-Martin d’Heres Cedex, France;(4) IRTSV, Laboratoire de Chimie et Biologie des Métaux (LCBM), CEA Grenoble 17 rue des Martyrs 38054, Grenoble cedex 9, France |
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Abstract: | In the present paper, the degradation of phenanthrene, a model polycyclic aromatic hydrocarbon compound, by the Mycobacterium strain 6PY1 was optimized in a biphasic culture medium. The optimization and modeling were performed using the design of
experiments methodology. The temperature, the silicone oil/mineral salts medium volume ratio, and the initial cell concentration,
were used as the central composite design parameters. In all experiments, the phenanthrene was degraded to undetectable levels.
Response surface methodology was successfully employed to derive an empirical model describing the rate and time of degradation
and to deduce the optimal degradation conditions. As a result of the optimization processes, the optimal responses for the
degradation rate, the volumetric degradation rate, and the 90% degradation time were estimated to be 0.172 mg h−1, 22 mg l−1 h−1, and 18 h, respectively. |
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Keywords: | Polycyclic aromatic hydrocarbons Phenanthrene Mycobacterium Biodegradation Biphasic culture Design of experiments |
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