Protein conformational changes induced by guanidine at predenaturational concentrations |
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| Authors: | E Bismuto G Irace |
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| Affiliation: | Section for Chemistry and Biochemistry, Faculty of Medicine, University of Naples, Italy. |
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| Abstract: | The nature of the molecular event that apomyoglobin undergoes at predenaturational concentrations of guanidine has been investigated by means of steady-state and multifrequency phase and modulation fluorometry. The results have been compared to those observed for liver alcohol dehydrogenase. From these studies has been hypothesized a different susceptibility of the distinct elements of secondary, super-secondary, and tertiary structure towards the denaturing action of guanidine at predenaturational concentrations. |
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