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Insect-resistant transgenic brinjal plants |
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| Authors: | Polumetla A Kumar Ajin Mandaokar Karra Sreenivasu Swarup K Chakrabarti Suman Bisaria Surat R Sharma Sarvjeet Kaur Rameshwar P Sharma |
| Institution: | (1) National Research Centre for Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, 110012, India |
| Abstract: | A synthetic cry1Ab gene coding for an insecticidal crystal protein (ICP) of Bacillus thuringiensis (Bt) was transferred to brinjal (eggplant) by cocultivating cotyledonary explants with Agrobacterium tumefaciens. Transformant plants resistant to kanamycin were regenerated. Hybridization experiments demonstrated gene integration and mRNA expression. Double-antibody sandwich ELISA analysis revealed Bt toxin protein expression in the transgenic plants. The expression resulted in a significant insecticidal activity of transgenic brinjal fruits against the larvae of fruit borer (Leucinodes orbonalis). The results also demonstrated that a synthetic gene based on monocot codon usage can be expressed in dicotyledonous plants for insect control. |
| Keywords: | Bacillus thuringiensis insecticidal crystal protein gene cry1Ab brinjal fruit borer Leucinodes orbonalis transgenic plants |
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